Unconventional myosin 1c as autoreactive cells apoptosis marker in multiple sclerosis

N.O. Negrych

Abstract


Comparative analysis to find the new markers of multiple sclerosis (MS) was conducted. The blood serum of multiple sclerosis patients and healthy donors was analyzed for 48 kDa unconventional myosin 1c (48 kDa Myo1c) isoform concentration. Materials and methods. Sixty one patients with multiple sclerosis and 20 healthy donors aged 19–57 years participated in the research. Methods of investigation: clinical (complaints, life and disease history, general and neurological examination), laboratory (identification of 48 kDa Myo1c in the blood serum by consequent serum protein sedimentation, electrophoresis and digital analysis of electrophoregrams; presence of Myo1c was confirmed by Western blot with anti-Myo1c antibodies). Results. In the blood serum of MS patients and healthy donors, 48 kDa Myo1c was identified. The group of MS patients had significantly higher level of serum 48 kDa Myo1c as compared to the control group. Considering the key role of T-lymphocytes in the autoreactive processes, higher level of serum 48 kDa Myo1c, that is present in great amounts in activated lymphocytes, results from increased apoptosis of these cells. The average age of patients with high levels of 48 kDa Myo1c was significantly lower in comparison to the group with decreased level of this protein. There were no significant correlations between 48 kDa Myo1c levels and sex detected. Conclusions. Myo1c plays an important role in MS pathogenesis, and its 48 kDa isoform may be used as an early diagnostic marker of MS, as well as quantitative indicator of apoptosis of activated autoreactive cells that is significant for the evaluation of disease-modifying treatment effectiveness.


Keywords


multiple sclerosis; apoptosis; marker; unconventional myosin 1c

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DOI: https://doi.org/10.22141/2224-0713.1.87.2017.96534

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