Investigation of the Association between ACE, AGT and FGB Gene Polymorphisms and Risk of Early Onset of Atherothrombotic Ischemic Stroke in Ukrainian Caucasian Population
Background. Atherothrombotic ischemic stroke accounts for about 50 % of all cases of ischemic strokes. It is a highly invalidating disease that affected not only older people, but also young adults, who can work, and children. Blood vessels damage can be linked with genetic changes in candidate genes, such as angiotensin-converting enzyme (ACE), angiotensinogen (AGT) and beta-fibrinogen (FGB). We aimed to investigate the association between I/D polymorphism (rs1799752) of ACE gene, T174M (rs4762) polymorphism of AGT gene and C-148T polymorphism of FGB gene with risk of early onset of atherothrombotic ischemic stroke in Ukrainian Caucasian population. Materials and methods. 78 cases (24 females and 54 males, mean age was 53.90 ± 9.43 years) and 55 controls (20 females and 35 males, mean age was 59.8 ± 14.8 years) were enrolled in this study. FGB and AGT polymorphisms were determined by polymerase chain reaction (PCR) followed by digestion with restriction enzymes. ACE polymorphism was determined by allele specific PCR method. Results. Risk assessment showed significant lower risk for I/D and D/D genotype of ACE gene carriers (odds ration (OR) = 0.303, 95% confidence interval (CI): 0.134–0.688; р < 0.05, and OR = 0.303, 95% CI: 0.134–0.688; р < 0.05, respectively) than for I/I genotype. Patients with -148CT genotype of FGB gene had a higher risk of developing ischemic stroke compared with -148CC carriers (OR = 2.452; 95% CI: 1.201–5.005; р < 0.05). No risk was obtained for polymorphism T174M of AGT gene. Conclusions. Our findings suggest that I/I genotype of ACE gene and C/T genotype of FGB gene are associated with early onset of atherothrombotic ischemic stroke in Ukrainian Caucasian population.
Full Text:PDF (Українська)
Fishchuk L.E. Genetic polymorphisms of the renin-angiotensin system in breast cancer patients./L.E. Fishchuk, N.G. Gorovenko.//Exp. Oncol.–2013.–№ 32(2).–P.101-104.
Association of angiotensin-converting enzyme (ACE) and fatty acid binding protein 2 (FABP2) genes polymorphism with type 2 diabetes mellitus in Northern India. / T.R.Sayed, F. Jalees, A. Faisal [et al.]. //J. of Renin-Angiotensin-Aldosteron Sys –2014.–.№15(4).–P.572-579.
Chanh N.T. Research of association T174M and M235T polymorphysms angiotensinogen gene with coronary heart disease in the population of the Rostov./ N.T. Chanh, T.P. Shkurat// Epydemyolohia.–2010.–T.11.–S.63-77
The influence of polymorphic variants of C-148T gene β chain of fibrinogen in warfarin dosing. / I.V. Malyarchuk, N.H. Horovenko, A.R. Babochkina, N.S. Osypenko//Krovoobih ta hemostaz.–2014.–V.№1-2.–S.115-118.
Vereshchahyn N.V. Principles of diagnosis and treatment of patients in the acute phase of stroke. / N.V. Vereshchahyn, M.A. Pyradov, Z.A. Suslyna //ConsiliumMedicum.–2001.–T3.–№5.–S.221-225.
Babych P.N. The application of modern statistical methods in the practice of clinical research. Message Two. The use of chi-square test / P.N. Babych,A.V. Chubenko, S.N. Lapach // Ukrayins'kyymedychnyychasopys. –2004.–№2.– S. 138-144.
ACE I/D genotype and I allele predicts ischemic stroke among males in South India. /M.Vijayan, R. Chinniah, P.M. Ravi [et al.] // Meta Gene.–2014.–№.2.–P.661-669.
Angiotensinogen gene gaplotype and hypertension interaction with ACE gene I allele. / C.T. Tsai, D. Fallin, F.T. Chiang [et al.] // Hypertension.–2003.–№41(1).–P.9-15.
Association of the ACE- II genotype with the risk of nephroticsyndrome in Pakistani children. / S. Shahid, A. Abid, S.Q. Mehdi [et al.] // Gene. 2012.–№493(1).–P.165-168.
Angiotensin II – indused process of angiogenesis is mediated by spleen tyrosine kinase via VEGF receptor phosphorilation. / K.B. Cuneyt., Y.S. Chi, A.Y. Fariborz // Am.J.Physiol. HeartCirc. Physiol.–2011.– №301.–P. 1043-1056.
Renin-angiotensin-aldosterone system blockade for cardiovascular disorders: current status./ T.K. Ma, K.K Kam, B.P. Yam, Yat-Yin Lam // Br.J.Pharmacol.–2010.–№.160(6).–P. 1273-1292.
Association of β-fibrinogen promoter gene polymorphism (-148C/T), hyperfibrinogenemia and ischemic stroke in young adult patients./I. Imran, L. Rusdi, I. Ponpon [et al.] // EgyptianJournalofMedicalHumanGenetics. – 2015.–№.16. –P. 11-17.
Fibrinogen beta-chain -C148T polymorphism is associated with increased fibrinogen, C-reactive protein and interleukin -6 in patients undergoing coronary artery bypass grafting./ E. Wypasek, E. Stepien, M. Kot[et al.]// Inflamation.–2012.–№35.–№2.–Р.429-435.
Copyright (c) 2017 INTERNATIONAL NEUROLOGICAL JOURNAL
This work is licensed under a Creative Commons Attribution 4.0 International License.
© Publishing House Zaslavsky, 1997-2018