The Effect of NeurogelTM Used with Bone Marrow Stem Cells Implantation on the Course of the Spasticity Syndrome after Experimental Spinal Cord Injury
Keywords:spinal cord injury, spasticity syndrome, restorative treatment, tissue neuroengineering, artificial tissue scaffold, bone marrow stem cells
Objective. To examine NeuroGelTM with bone marrow stem cells (BMSC) implantation on the dynamics of rat’s paretic hind limb spasticity after experimental spinal cord injury. Materials and methods. Animals: outbred albino rats (male, 5.5 months, 250 g); experimental groups: 1 — spinal cord injury (n = 16); 2 — spinal cord injury + immediate homotopical transplantation of NeuroGelTM (n = 20); 3 — spinal cord injury + analogous transplantation of NeuroGelTM in association with adult mouse BMSC (n = 16). The model of injury was left-side spinal cord hemisection at Т11; the duration of observation was 28 weeks; the ipsilateral hind limb (IHL) function indicator (FI) and spasticity indicator (SI) were determined by the Вasso — Вeattie — Вresnahan scale (BBB) and Ashworth scale, respectively. Results. At the 28th week of the experiment IHL SI in group 1 was 2.5 ± 0.4 points at Ashworth scale, in group 2 and 3 — 1.7 ± 0.2 and 1.7 ± 0.3, respectively. Significant difference between the values of the IHL SI in groups 1 and 2 was found on the 7th day, during 5–7th and 12–24th week, between groups 1 and 3 — on the 7th day, during the 7–8th and at the 16th week. The maximum difference between groups 2 and 3 IHL SI value was observed at the 12th week (р = 0.059). The dynamics of IHL SI average value in groups 2 and group 3 was similar, except for the lack of significant growth during the 3rd week, and the presence of significant growth during the 3–4th months. Unlike group 1, in groups 2 and 3 there was observed negative correlation between individual IHL FI and SI values in each of the observation terms combined with the strong positive correlation between these values along the observation period. Conclusion. BMSC xenotransplantation using the NeuroGelTM does not lead to significant changes in the spasticity level compared to the isolated implantation using the NeuroGelTM, but significantly alters the dynamic pattern of spinal injury complications.
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