The Effect of Implantation of Neurogeltm Used with Xenogenic Bone Marrow Stem Cells on Motor Function Recovery after Experimental Spinal Cord Injury
Keywords:spinal cord injury, recovery treatment, tissue neuroengineering, artificial tissue scaffold, bone marrow stem cells
Objective. To examine NeuroGelTM with bone marrow stem cells (BMSC) implantation on rat’s hind limb motor function recovery after experimental spinal cord injury. Materials and methods. Animals: outbred albino male rats (5.5 months, 250 g); experimental groups: 1st — spinal cord injury only (n = 16); 2nd — spinal cord injury + immediate homotopical transplantation of NeuroGelTM (n = 20); 3rd — spinal cord injury + analogous transplantation of NeuroGelTM in association with adult mouse BMSC (n = 16). Model of injury: left-side spinal cord hemisection at Т11; the ipsilateral hindlimb function indicator (IHL FI) was detected using the Вasso — Вeattie — Вresnahan scale (BBB). Results. Xenotransplantation of the BMSC together with NeuroGelTM extends the period, but reduces the intensity of IHL FI growth in the acute and early period of traumatic process, prolongs a phase of significant IHL FI growth to the 6th month inclusive. In group 1 significant (p < 0.05) increase of IHL FI was observed during 3rd — 4th week only, in group 2 — during 1st — 2nd and 5th — 6th week, in group 3 — during 1st — 2nd, 4th — 5th and 8th — 24th week. At the 28th week in group 1 IHL FI was 1.6 ± 0.5 points, in group 2 — 8.4 ± 0.9 points, in group 3 — 11.0 ± 1.5 points by ВВВ scale. Significant difference in IHL FI between groups 2 and 1 was being registered for 2nd — 28th weeks (р < 0.001), between groups 3 and 1 — during 1st — 28th week (р ≤ 0.02). The maximal difference between groups 2 and 3 in favor of the first one was noted on the 24th week (р = 0.055). Conclusion. BMSC-xenotransplantation in association with NeuroGelTM changes the dynamics of the paretic limb function recovery, it provides a tendency towards intensification of the NeuroGelTM positive impact on the course of the spinal cord injury.
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