DOI: https://doi.org/10.22141/2224-0713.16.5.2020.209246

A prognostic value of the study of the exposure of glycosyl determinants to IgG molecules in the blood serum of patients with multiple sclerosis depending on the performed drug therapy

M.O. Boichuk, T.I. Nehrych, R.O. Bilyy

Abstract


Background. The definition of antibodies to the body’s own antigens is of great importance in autoimmune processes in multiple sclerosis. The functions of immunoglobulins (Ig) are determined by the composition of the biomolecules of glycan residues attached to the N-glycosylated domain in the crystallizing fragment (Fc region) of the immunoglobulin heavy chains. The changes in the structure of glycans are believed to be able to affect the functioning of Ig. So, the presence or absence of certain carbohydrate residues in the composition of antibodies is associated with their pro- or anti-inflammatory properties. In turn, this modifies the course of inflammatory reactions in autoimmune diseases and can determine the activity of multiple sclerosis. Therefore, studies of structural changes in the blood serum Ig of patients with multiple sclerosis is of great clinical importance. Materials and methods. The serum samples of 6 cohorts of patients with multiple sclerosis was sdudied depending on the group of the drug of the disease-modifying therapy that the patients were taking. Namely, 17 patients, prior to the time of blood sampling for these studies, had never received any therapy; 15 patients with multiple sclerosis received disease-modifying therapy with drugs from the interferon group β-1b; 12 people received pulse therapy with methylpredni-solone at a dose of 1000 mg for 5–7 days; 12 patients with multiple sclerosis of different sexes and ages received therapy with drugs from the group of monoclonal antibodies, namely ocrelizumab at a dose of 600 mg once per 6 months; 9 people received glatiramer acetate at a dose of 40 mg three times a week; 10 patients with multiple sclerosis were treated with teriflunomide at a dose of 14 mg
once a day; there were 23 healthy individuals. Results. The results of the studies demonstrated that various types of therapy impact the change in the immunoglobulins glycosyl determinants. Conclusions. Glycan markers can be used as high-sensitive and specific biomarkers for diagnosing multiple sclerosis and predicting its course.


Keywords


multiple sclerosis; IgG; glycosylation

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