DOI: https://doi.org/10.22141/2224-0713.16.3.2020.203446

Investigation of antibody glycosylation in serum and cerebrospinal fluid of patients with multiple sclerosis as a biomarker in the disease

К.М. Hychka, T.I. Nehrych, R.O. Bilyy

Abstract


Background. The purpose of this work was to improve the diagnosis of multiple sclerosis (MS) and determination of new biomarkers of this disease. Materials and methods. In this work, we are investigating the aspects of changes of human glycosyl determinants in multiple sclerosis that have not been previously investigated. Therefore, we have focused on two main areas — studies of antibody glycosylation in cerebrospinal fluid (CSF) and its comparison with the serum antibodies in patients with MS and determination of their nature. It was important for us to analyze the clinical course of multiple sclerosis in patients to identify possible clinical patterns. Therefore, we carried out a thorough analysis of clinical records of 7 patients; we have taken a serum and cerebrospinal fluid for immunological studies in these patients. The paper presents the results of the analysis. Results. The results of these studies showed an increase of exposure of fucosyl residue — targets of AAL on immunoglobulins G (IgG) isolated from CSF, as well as an increase of exposure level of targets of LCA (specific to cerebral cortex residues of IgG N-glycans). At the same time, we observed a significantly lower binding level of sialospecific lectins SNA and PSqL in the CSF that indicates a lower degree of sialylation of IgG molecules in the CSF. Therefore, immunoglobulins G in CSF have more pronounced pro-inflammatory effects compared to IgG in patients’ blood serum. Conclusions. The study of antibody glycosylation in the CSF and serum of patients with MS is extremely important as a key link in the pathogenesis of the disease and as a reliable biomarker of this pathological condition.


Keywords


multiple sclerosis; biomarker; glycosylation; immunoglobulins; cerebrospinal fluid

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