Influence of interleukin-10 on factors of immune and antioxidant protection of the brain, thymic function and behaviour in the cuprizone mouse model of demyelination
Background. The role of macrophages, Tlymphocytes and factors of oxidative stress in damage to the nerve cells of the brain leading to disruption of their functioning is known. Thymic hormone thymulin exhibits immunomodulating properties whereas interleukin10 possesses pronounced antiinflammatory properties and affects neurogenesis. This work aimed to study changes in the content of macrophages, Tlymphocytes, malondialdehyde, activity of antioxidant enzymes in the brain, blood thymulin levels, and behavioral reactions in mice with cuprizone demyelination model treated with recombinant human IL10 (rhIL10). Materials and methods. Adult 129/Sv mice received cuprizone daily with food for 3 weeks. Starting from day 7 of cuprizone diet, rhIL10 was administered intraperitoneally at a dose of 5 µg/kg (total of 3 injections, with an interval of 3 days). Results. The number of latexphagocyting macrophages, CD3+ Tcells and malondialdehyde content increased while the activity of antioxidant enzymes decreased in the brain of cuprizonetreated mice. Following rhIL10 injections, we observed a decrease in the number of CD3+ Tcells and macrophage activity and an increase in the superoxide dismutase, catalase and glutathione peroxidase activities. Besides, the thymulin blood level increased. Interestingly, after cytokine injection we observed an increase in the horizontal locomotor, emotional and exploratory activities, being decreased by cuprizone. Conclusions. Locomotor, emotional and exploratory activity tests showed that rhIL10 improved the central nervous system functioning in the cuprizonetreated mice. RhIL10 effect in mice on cuprizone diet was mainly associated with changes in the number of brain Tlymphocytes, the activity of macrophages and antioxidant enzymes, as well as the endocrine function of the thymus. Interleukin10 or agents/approaches enhancing its synthesis in the central nervous system might be promising in the demyelinating pathology treatment schemes.
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