Influence of interleukin-10 on factors of immune and antioxidant protection of the brain, thymic function and behaviour in the cuprizone mouse model of demyelination

I.F. Labunets, A.Ye. Rodnichenko, N.O. Utko, Ya.O. Pokholenko


Background. The role of macrophages, T­lymphocytes and factors of oxidative stress in damage to the nerve cells of the brain leading to disruption of their functioning is known. Thymic hormone thymulin exhibits immunomodulating properties whereas interleukin­10 possesses pronounced anti­inflammatory properties and affects neurogenesis. This work aimed to study changes in the content of macrophages, T­lymphocytes, malondialdehyde, activi­ty of antioxidant enzymes in the brain, blood thymulin levels, and behavioral reactions in mice with cuprizone demyelination model treated with recombinant human IL­10 (rhIL­10). Materials and methods. Adult 129/Sv mice received cuprizone daily with food for 3 weeks. Starting from day 7 of cuprizone diet, rhIL­10 was admini­stered intraperitoneally at a dose of 5 µg/kg (total of 3 injections, with an interval of 3 days). Results. The number of latex­phagocyting macrophages, CD3+ T­cells and malondialdehyde content increased while the activity of antioxidant enzymes decreased in the brain of cuprizone­treated mice. Following rhIL­10 injections, we observed a decrease in the number of CD3+ T­cells and macrophage activity and an increase in the superoxide dismutase, catalase and glutathione peroxidase activities. Besides, the thymulin blood level increased. Interestingly, after cytokine injection we observed an increase in the horizontal locomotor, emotional and exploratory acti­vities, being decreased by cuprizone. Conclusions. Locomotor, emotional and exploratory activity tests showed that rhIL­10 improved the central nervous system functioning in the cuprizone­treated mice. RhIL­10 effect in mice on cuprizone diet was mainly associated with changes in the number of brain T­lymphocytes, the acti­vity of macrophages and antioxidant enzymes, as well as the endocrine function of the thymus. Interleukin­10 or agents/approaches enhancing its synthesis in the central nervous system might be promising in the demyelinating pathology treatment schemes.


neurotoxin cuprizone; interleukin-10; macrophages, T-cells and antioxidant enzymes of the brain; thymulin; behavioral reactions


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