Changes of thymic endocrine function, brain macrophages and T-lymphocytes in mice of different ages after administration of neurotoxin cuprizone and cytokine

I.F. Labunets


Background. Macrophages and T­lymphocytes are well known to be involved in the damage to the brain nervous cells. Thymic hormone thymulin exhibits immunomodulatory and anti­inflammatory effects. The age­related changes in the activity of brain macrophages, T­lymphocytes and thymus can affect their response to the toxic and regulatory factors. The purpose was to study the changes in blood thymulin level and amount of brain macrophages and T­lymphocytes in young and aged mice after administration of neurotoxin cuprizone and recombinant human leukemia inhibitory factor (rhLIF). Materials and methods. The 129/Sv mice received cuprizone with food daily for 3 weeks. From day 8 of cuprizone diet, the rhLIF was injected daily (50 µg/kg). Results. Increase of blood thymulin level after 7­day cuprizone diet was more significant in young versus aged mice. Hormone level decreased after 3­week cuprizone treatment only in young mice. After 7 days, the number of brain macrophages in young mice increased, while in aged mice — only after 3 weeks. In cuprizone­treated mice of both age groups (especially in young), the number of brain T­cells increased. RhLIF injections increased blood thymulin level and decreased the number of brain T­cells in young cuprizone­treated mice, while number of brain macrophages decreased only in aged mice. Conclusions. The effect of both cuprizone and rhLIF on thymic endocrine function and the number of brain macrophages and T­lymphocytes in mice is age­dependent.


thymulin; brain macrophages and T-cells; cuprizone; leukemia-inhibitory factor; age


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